The CBSE Class 12 Biotechnology Syllabus 2024-25 provides a detailed outline for students, covering essential topics like Protein and Gene Manipulation, Cell Culture, and Genetic Manipulation. It helps students understand core biotechnology concepts and prepares them for both theoretical exams and practicals.
CBSE Class 12 Bio-Technology Syllabus 2024-25
CBSE has also released the official syllabus PDF for Class 12 Biotechnology for the 2024-25 session. This document includes important information like the exam pattern, marking scheme, and detailed topic breakdown, ensuring students have all the resources they need for effective preparation.
Units and Marks Distribution
| Unit | Marks |
|---|---|
| Unit V: Protein and Gene Manipulation | 40 |
| Unit VI: Cell Culture and Genetic Manipulation | 30 |
| Practicals | 30 |
Unit V: Protein and Gene Manipulation (40 Marks)
Chapter 1: Recombinant DNA Technology
- Introduction to Recombinant DNA Technology.
- Tools of Recombinant DNA technology.
- Making an rDNA molecule.
- Introduction of recombinant DNA into host cells.
- Identification of recombinants.
- Polymerase Chain Reaction (PCR).
- DNA Sequencing.
Chapter 2: Protein Structure and Engineering
- Introduction to proteins.
- Structure-function relationship in proteins.
- Characterization of proteins.
- Protein-based products.
- Designing proteins (Protein Engineering).
Chapter 3: Genomics, Proteomics, and Bioinformatics
- Gene prediction and counting.
- Genome similarity.
- SNPs (Single Nucleotide Polymorphisms) and Comparative genomics.
- Functional genomics.
- Proteomics.
- Information sources and analysis using bioinformatics tools.
Unit VI: Cell Culture and Genetic Manipulation (30 Marks)
Chapter 1: Microbial Cell Culture and its Applications
- Introduction to microbial culture.
- Microbial nutrition and culture techniques.
- Measurement and kinetics of microbial growth.
- Isolation of microbial products.
- Strain isolation and improvement.
- Applications of microbial culture technology.
Chapter 2: Plant Cell Culture and Applications
- Introduction to cell and tissue culture techniques.
- Applications of cell and tissue culture.
- Transgenic plants with beneficial traits.
- Biosafety of transgenic plants.
Chapter 3: Animal Cell Culture and Applications
- Introduction to animal cell culture techniques.
- Applications of animal cell culture.
- Stem cell technology.
Practicals (30 Marks)
Experiments
- Use of special equipment in biotechnology experiments.
- Isolation of bacterial plasmid DNA.
- Detection of DNA by gel electrophoresis.
- Estimation of DNA by UV spectroscopy.
- Isolation of bacteria from curd & staining of bacteria.
- Cell viability assay using Evan’s blue dye exclusion method.
- Data retrieval and database search using NCBI. Download and analyze a DNA and protein sequence.
- Reading of a DNA sequencing gel.
- Project work.
Scheme of Evaluation (Practical)
| Component | Marks |
|---|---|
| Two experiments (including one computer-based practical) | 6 + 6 |
| Practical record | 4 |
| Viva on practical | 4 |
| Project work write-up | 5 |
| Viva on project | 5 |
| Total | 30 |
Assessment Areas (Theory) 2024-25
| Competencies | Weightage |
|---|---|
| Demonstrate Knowledge and Understanding | 50% |
| Application of Knowledge / Concepts | 30% |
| Analyze, Evaluate, and Create | 20% |
Typology of Questions:
- Very Short Answer (VSA) including MCQs.
- Assertion-Reasoning type questions.
- Short Answer (SA).
- Long Answer I (LA-I).
- Long Answer II (LA-II).
- Source-based, Case-based, Passage-based, Integrated assessment questions.
Note: An internal choice of around 33% will be provided.
Suggestive Verbs for Competencies
- Demonstrate Knowledge and Understanding: State, name, list, identify, define, suggest, describe, outline, summarize.
- Application of Knowledge/Concepts: Calculate, illustrate, show, adapt, explain, distinguish.
- Analyze, Evaluate, and Create: Interpret, analyze, compare, contrast, examine, evaluate, discuss, construct.
CBSE Class 12 Bio-Technology Syllabus 2024-25 PDF Download
For more details and to download the CBSE Class 12 Bio-Technology Syllabus 2024-25 in PDF format, visit the official CBSE website or download it via the link given below. The syllabus outlines the course structure, marking scheme, and preparation guidelines for students pursuing biotechnology in Class 12.
CBSE Class 12 Bio-Technology Syllabus 2024-25-Click Here To Download PDF
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